15 Sept 2021 Published by Journal of Ethnopharmacology (IF 4.36) © Elsevier B.V.
In this study, researchers studied immune profiles and efficacy of aqueous extracts from cultivated Cistanche deserticola Y. C. Ma via animal models and vitro strategy, and evaluated the activation mechanism of dendritic cells under the effect of aqueous extracts.
Followed by hot water extraction and ethanol precipitation, the polysaccharide adjuvants of cultivated Cistanche deserticola Y. C. Ma aqueous extracts was prepared. In the vivo (ICR mice) experiments, the polysaccharide adjuvants was designed as gradient in dose to separate in different groups. The results showed that polysaccharide adjuvants increased splenocyte proliferation about 2 times as compared with the control group, as well as significantly promoted the activated T cell response. Besides, polysaccharide adjuvants stimulated and activated the initial immune cells (dendritic cells) while inhabited regulatory T cells. For the study on cytotoxicity, researchers found that the cell viability and proliferation response of dendritic cells and splenocytes were not inhibited when the concentration of polysaccharide adjuvants was in 10-1600 μg/mL. As well, it was proved that there was no endotoxin contamination through unsing antibiotic (used to exclude endotoxin contamination) on Cistanche deserticola Y. C. Ma aqueous extracts.
The researchers concluded that Cistanche deserticola Y. C. Ma aqueous extracts could be made as a kind of polysaccharide adjuvants to effectively enhance the proliferation of T cells and increase the production of immunomodulators, as well as improved antigen targeting onto dendritic cells. On the researchers’ opinion, vaccine adjuvants against infectious disease could be designed according to specific adjuvant propertie of Cistanche deserticola Y. C. Ma aqueous extracts.
Feng, Yang, X., Weng, X., Wang, B., & Zhang, A. (2021). Aqueous extracts from cultivated Cistanche deserticola Y.C. Ma as polysaccharide adjuvant promote immune responses via facilitating dendritic cell activation. Journal of Ethnopharmacology, 277, 114256–114256.
